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Cosco, E. ; Arus, B.A. ; Spearman, A.L.* ; Atallah, T.L.* ; Lim, I.* ; Leland, O.S.* ; Caram, J.R.* ; Bischof, T.S. ; Bruns, O.T. ; Sletten, E.M.*

Bright chromenylium polymethine dyes enable fast, four-color in vi-vo imaging with shortwave infrared detection.

J. Am. Chem. Soc. 143, 6836-6846 (2021)
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Optical imaging within the shortwave infrared (SWIR, 1000-2000 nm) region of the electromagnetic spectrum has enabled high-resolution and high-contrast imaging in mice, non-invasively. Polymethine dyes, with their narrow absorption spectra and high absorption coefficients, are optimal probes for fast and multiplexed SWIR imaging. Here, we expand upon the multiplexing capabilities in SWIR imaging by obtaining brighter polymethine dyes with varied excitation wavelengths spaced throughout the near-infrared (700-1000 nm) region. Building on the flavylium polymethine dye scaffold, we explored derivatives with functional group substitution at the 2-position, deemed chromenylium polymethine dyes. The reported dyes have reduced nonradiative rates and enhanced emissive properties, enabling non-invasive imaging in mice in a single color at 300 fps and in three colors at 100 fps. Combined with polymethine dyes containing a red-shifted julolidine flavylium heterocycle and indocyanine green, distinct channels with well-separated excitation wavelengths provide non-invasive video-rate in vivo imaging in four colors.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Cyanine; Fluorophores; Surgery
ISSN (print) / ISBN 0002-7863
e-ISSN 1520-5126
Quellenangaben Volume: 143, Issue: 18, Pages: 6836-6846 Article Number: , Supplement: ,
Publisher American Chemical Society (ACS)
Publishing Place 1155 16th St, Nw, Washington, Dc 20036 Usa
Reviewing status Peer reviewed
Institute(s) Helmholtz Pioneer Campus (HPC)
Grants UCLA
Foote Family
Alfred P. Sloan Foundation
Emmy-Noether-Program of DFG
Helmholtz Pioneer Campus Institute for Biomedical Engineering