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In-gel proteasome assay to determine the activity, amount, and composition of proteasome complexes from mammalian cells or tissues.

STAR Protoc. 2:100526 (2021)
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This protocol describes an easy and reliable in-gel proteasome assay to quantify the activity and composition of different proteasome complexes in cells and tissues. The assay works well with limited amounts of total cell protein lysates. Although this assay is optimized specifically for the proteasome chymotrypsin-like activity, it can be expanded to other proteasome activities as well. Using antibodies that detect distinct proteasome subunits or regulators, we can determine the composition and relative quantity of active proteasome complexes. For complete details on the use and execution of this protocol, please refer to Meul et al. (2020).
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Antibody ; Cell Biology ; Molecular Biology ; Protein Biochemistry
e-ISSN 2666-1667
Zeitschrift STAR Protocols
Quellenangaben Band: 2, Heft: 2, Seiten: , Artikelnummer: 100526 Supplement: ,
Begutachtungsstatus Peer reviewed