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Yudintceva, N.* ; Mikhailova, N.* ; Bobkov, D.* ; Yakovleva, L.* ; Nikolaev, B.* ; Krasavina, D.* ; Muraviov, A.* ; Vinogradova, T.* ; Yablonskiy, P.* ; Samusenko, I.* ; Ryzhov, V.* ; Deriglazov, V.* ; Marchenko, Y.* ; Multhoff, G.* ; Klapproth, A. ; Li, W.B. ; Nayak, B.* ; Sonawane, A.* ; Shevtsov, M.*

Evaluation of the biodistribution of mesenchymal stem cells in a preclinical renal tuberculosis model by nonlinear magnetic response measurements.

Front. Physics 9, DOI: 10.3389/fphy.2021.625622 (2021)
Verlagsversion Forschungsdaten DOI
Open Access Gold
Creative Commons Lizenzvertrag
Bone-marrow derived mesenchymal stem cells (MSCs) exert anti-tuberculosis effects due to their potential to repair damaged tissues and modulate inflammatory immune responses. MSCs were reported to be recruited to the Mycobacterium tuberculosis (Mtb) affected sites in the organism. However, due to limitations of presently applied in vivo imaging techniques the trafficking and biodistribution of MSCs in Mtb-infected organisms is not possible. In the current study MSCs were labeled with superparamagnetic iron oxide nanoparticles (SPIONs) as a negative MR contrast agent for imaging the biodistribution of MSCs in vivo. Trafficking of SPIONs-labeled MSCs was analyzed in a preclinical model of renal tuberculosis in male Chinchilla rabbits (n = 18) following intravenous administration on the days 0, 2, 3, and 7 employing a highly sensitive method of non-linear longitudinal magnetic response (NLR-M-2) measurements. Within 48 h after injection, nanoparticle-labeled MSCs accumulated predominantly in lung, spleen, liver tissues, and paratracheal lymph nodes with subsequent decrease over the observation period of 7 days. The recruitment of MSCs to Mtb-affected organs was further proven by immunohistological analysis. NLR-M-2 allowed the detection of SPIONs-labeled cells at low concentrations in different organs and tissues giving insights of in vivo mesenchymal stem cells trafficking in organism after TB infection.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Renal Tuberculosis ; Mesenchymal Stem Cells ; Biodistribution ; Superparamagnetic Iron Oxide Nanoparticles ; Spions ; Non-linear Magnetic Response; Iron-oxide Nanoparticles; Cellular-therapy; Stromal Cells; Rabbit; Transplantation; Infusion; Tracking; Tract
ISSN (print) / ISBN 2296-424X
e-ISSN 2296-424X
Zeitschrift Frontiers in Physics
Quellenangaben Band: 9 Heft: , Seiten: , Artikelnummer: , Supplement: ,
Verlag Frontiers
Verlagsort Avenue Du Tribunal Federal 34, Lausanne, Ch-1015, Switzerland
Begutachtungsstatus Peer reviewed
Förderungen Department of Science and Technology (DST) (International Bilateral Cooperation Division), Government of India
Ministry of Science and Higher Education
Technische Universitat Munchen (TUM) within the DFG funding programme Open Access Publishing
Russian Foundation for Basic Research
German Research Foundation DFG