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Greulich, F.* ; Mechtidou, A. ; Horn, T. ; Uhlenhaut, N.H.

Protocol for using heterologous spike-ins to normalize for technical variation in chromatin immunoprecipitation.

STAR Protoc. 2:100609 (2021)
Verlagsversion DOI
Open Access Gold
Creative Commons Lizenzvertrag
Quantifying differential genome occupancy by chromatin immunoprecipitation (ChIP) remains challenging due to variation in chromatin fragmentation, immunoprecipitation efficiencies, and intertube variability. In this protocol, we add heterologous spike-ins from Drosophila chromatin as an internal control to the mice chromatin before immunoprecipitation to normalize for technical variation in ChIP-qPCR or ChIP-seq. The choice of spike-in depends on the evolutionary conservation of the protein of interest and the antibody used. For complete details on the use and execution of this protocol, please refer to Greulich et al. (2021).
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Cell Biology ; Chipseq ; Chromatin Immunoprecipitation (chip) ; Molecular Biology ; Sequence Analysis
e-ISSN 2666-1667
Zeitschrift STAR Protocols
Quellenangaben Band: 2, Heft: 3, Seiten: , Artikelnummer: 100609 Supplement: ,
Begutachtungsstatus Peer reviewed