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Schranner, D.* ; Schönfelder, M.* ; Römisch-Margl, W. ; Scherr, J.* ; Schlegel, J.* ; Zelger, O.* ; Riermeier, A.* ; Kaps, S.* ; Prehn, C. ; Adamski, J. ; Söhnlein, Q.* ; Stocker, F.* ; Kreuzpointner, F.* ; Halle, M.* ; Kastenmüller, G. ; Wackerhage, H.*

Physiological extremes of the human blood metabolome: A metabolomics analysis of highly glycolytic, oxidative, and anabolic athletes.

Physiol. Rep. 9:e14885 (2021)
Publ. Version/Full Text Research data DOI
Open Access Gold
Creative Commons Lizenzvertrag
Human metabolism is highly variable. At one end of the spectrum, defects of enzymes, transporters, and metabolic regulation result in metabolic diseases such as diabetes mellitus or inborn errors of metabolism. At the other end of the spectrum, favorable genetics and years of training combine to result in physiologically extreme forms of metabolism in athletes. Here, we investigated how the highly glycolytic metabolism of sprinters, highly oxidative metabolism of endurance athletes, and highly anabolic metabolism of natural bodybuilders affect their serum metabolome at rest and after a bout of exercise to exhaustion. We used targeted mass spectrometry-based metabolomics to measure the serum concentrations of 151 metabolites and 43 metabolite ratios or sums in 15 competitive male athletes (6 endurance athletes, 5 sprinters, and 4 natural bodybuilders) and 4 untrained control subjects at fasted rest and 5 minutes after a maximum graded bicycle test to exhaustion. The analysis of all 194 metabolite concentrations, ratios and sums revealed that natural bodybuilders and endurance athletes had overall different metabolite profiles, whereas sprinters and untrained controls were more similar. Specifically, natural bodybuilders had 1.5 to 1.8-fold higher concentrations of specific phosphatidylcholines and lower levels of branched chain amino acids than all other subjects. Endurance athletes had 1.4-fold higher levels of a metabolite ratio showing the activity of carnitine-palmitoyl-transferase I and 1.4-fold lower levels of various alkyl-acyl-phosphatidylcholines. When we compared the effect of exercise between groups, endurance athletes showed 1.3-fold higher increases of hexose and of tetradecenoylcarnitine (C14:1). In summary, physiologically extreme metabolic capacities of endurance athletes and natural bodybuilders are associated with unique blood metabolite concentrations, ratios, and sums at rest and after exercise. Our results suggest that long-term specific training, along with genetics and other athlete-specific factors systematically change metabolite concentrations at rest and after exercise.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Athlete ; Energy Metabolism ; Exercise Biomarker ; Exercise Phenotype
ISSN (print) / ISBN 2051-817X
e-ISSN 2051-817X
Quellenangaben Volume: 9, Issue: 12, Pages: , Article Number: e14885 Supplement: ,
Publisher Wiley
Reviewing status Peer reviewed
Institute(s) Institute of Computational Biology (ICB)
Molekulare Endokrinologie und Metabolismus (MEM)
Core Facility Metabolomics & Proteomics (CF-MPC)
Studienstiftung des Deutschen Volkes
Bundesministerium für Bildung und Forschung