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Seiringer, P. ; Eyerich, S. ; Eyerich, K. ; Dittlein, D. ; Pilz, A.C. ; Scala, E.* ; Ring, J.* ; Behrendt, H. ; Cavani, A.* ; Traidl-Hoffmann, C.

Keratinocytes regulate the threshold of inflammation by inhibiting T cell effector functions.

Cells 10:1606 (2021)
Verlagsversion Forschungsdaten DOI
Open Access Gold
Creative Commons Lizenzvertrag
Whilst the importance of keratinocytes as a first-line defense has been widely investigated, little is known about their interactions with non-resident immune cells. In this study, the impact of human keratinocytes on T cell effector functions was analyzed in an antigen-specific in vitro model of allergic contact dermatitis (ACD) to nickel sulfate. Keratinocytes partially inhibited T cell proliferation and cytokine production. This effect was dependent on the keratinocyte/T cell ratio and was partially reversible by increasing the number of autologous dendritic cells. The inhibition of T cell proliferation by keratinocytes was independent of the T cell subtype and antigen presentation by different professional antigen-presenting cells. Autologous and heterologous keratinocytes showed comparable effects, while the fixation of keratinocytes with paraformaldehyde abrogated the immunosuppressive effect. The separation of keratinocytes and T cells by a transwell chamber, as well as a cell-free keratinocyte supernatant, inhibited T cell effector functions to the same amount as directly co-cultured keratinocytes, thus proving that soluble factor/s account for the observed suppressive effects. In conclusion, keratinocytes critically control the threshold of inflammatory processes in the skin by inhibiting T cell proliferation and cytokine production.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter T Cell Effector Functions ; T Cells ; Keratinocytes ; Skin Barrier ; Skin Immune Homeostasis; Dendritic Cells; Atopic-dermatitis; Lymphocytes; Expression; Antigen; Nickel; Skin; Activation; Crosstalk; Induction
ISSN (print) / ISBN 2073-4409
e-ISSN 2073-4409
Zeitschrift Cells
Quellenangaben Band: 10, Heft: 7, Seiten: , Artikelnummer: 1606 Supplement: ,
Verlag MDPI
Verlagsort Basel
Institut(e) Institute for Allergy Research (IAF)
Institute of Environmental Medicine (IEM)