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van der Vorst, E.P.C.* ; Pepe, M. ; Peters, L.J.F.* ; Haberbosch, M.* ; Jansen, Y.* ; Nauman, R.* ; Stathopoulos, G.T. ; Weber, C.* ; Bidzhekov, K.*

Transcriptome signature of miRNA-26b KO mouse model suggests novel targets.

BMC Genom Data 22:23 (2021)
Verlagsversion Forschungsdaten DOI
Creative Commons Lizenzvertrag
BACKGROUND: MicroRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. One of the miRNAs that has been shown to play a role in various pathologies like cancer, neurological disorders and cardiovascular diseases is miRNA-26b. However, these studies only demonstrated rather ambiguous associations without revealing a causal relationship. Therefore, the aim of this study is to establish and validate a mouse model which enables the elucidation of the exact role of miRNA-26b in various pathologies. RESULTS: A miRNA-26b-deficient mouse model was established using homologous recombination and validated using PCR. miRNA-26b-deficient mice did not show any physiological abnormalities and no effects on systemic lipid levels, blood parameters or tissue leukocytes. Using next generation sequencing, the gene expression patterns in miRNA-26b-deficient mice were analyzed and compared to wild type controls. This supported the already suggested role of miRNA-26b in cancer and neurological processes, but also revealed novel associations of miRNA-26b with thermogenesis and allergic reactions. In addition, detailed analysis identified several genes that seem to be highly regulated by miRNA-26b, which are linked to the same pathological conditions, further confirming the role of miRNA-26b in these pathologies and providing a strong validation of our mouse model. CONCLUSIONS: miRNA-26b plays an important role in various pathologies, although causal relationships still have to be established. The described mouse model of miRNA-26b deficiency is a crucial first step towards the identification of the exact role of miRNA-26b in various diseases that could identify miRNA-26b as a promising novel diagnostic or even therapeutic target in a broad range of pathologies.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Allergic Reactions ; Cancer ; Cardiovascular Disease ; Knock-out Mouse Model ; Microrna-26b ; Neurological Disorders ; Next Generation Sequencing ; Thermogenesis; Microrna Expression; Wnk Kinases; Survival; Dectin-2; Growth
ISSN (print) / ISBN 2730-6844
e-ISSN 2730-6844
Zeitschrift BMC Genomic Data
Quellenangaben Band: 22, Heft: 1, Seiten: , Artikelnummer: 23 Supplement: ,
Verlag BioMed Central
Verlagsort Campus, 4 Crinan St, London N1 9xw, England
Begutachtungsstatus Peer reviewed
Förderungen H2020 European Research Council