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Wietrzynski, W. ; Traverso, E.* ; Wollman, F.A.* ; Wostrikoff, K.*

The state of oligomerization of Rubisco controls the rate of synthesis of the Rubisco large subunit in Chlamydomonas reinhardtii.

Plant Cell 33, 1706-1727 (2021)
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Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) is present in all photosynthetic organisms and is a key enzyme for photosynthesis-driven life on Earth. Its most prominent form is a hetero-oligomer in which small subunits (SSU) stabilize the core of the enzyme built from large subunits (LSU), yielding, after a chaperone-Assisted multistep assembly process, an LSU8SSU8 hexadecameric holoenzyme. Here we use Chlamydomonas reinhardtii and a combination of site-directed mutants to dissect the multistep biogenesis pathway of Rubisco in vivo. We identify assembly intermediates, in two of which LSU are associated with the RAF1 chaperone. Using genetic and biochemical approaches we further unravel a major regulation process during Rubisco biogenesis, in which LSU translation is controlled by its ability to assemble with the SSU, via the mechanism of control by epistasy of synthesis (CES). Altogether this leads us to propose a model whereby the last assembly intermediate, an LSU8-RAF1 complex, provides the platform for SSU binding to form the Rubisco enzyme, and when SSU is not available, converts to a key regulatory form that exerts negative feedback on the initiation of LSU translation.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Ribulose-bisphosphate Carboxylase; Bundle-sheath Defective2; Ribulose-1,5-bisphosphate Carboxylase/oxygenase; Messenger-rna; Assembly Chaperone; Crystal-structure; Oxidative Stress; Gene-expression; Mesophyll-cells; Cytochrome F
ISSN (print) / ISBN 1040-4651
e-ISSN 1532-298X
Journal The Plant cell
Quellenangaben Volume: 33, Issue: 5, Pages: 1706-1727 Article Number: , Supplement: ,
Publisher American Society of Plant Biologists (ASPB)
Publishing Place Journals Dept, 2001 Evans Rd, Cary, Nc 27513 Usa
Reviewing status Peer reviewed
Institute(s) Helmholtz Pioneer Campus (HPC)
Grants PICS 5462
Complexite du Vivant
Labex Dynamo
Sorbonne Universite (SU)
Centre National de la Recherche Scientifique (CNRS)