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Single-molecule techniques to study chromatin.

Front. Cell Dev. Biol. 9:699771 (2021)
Verlagsversion DOI
Open Access Gold
Creative Commons Lizenzvertrag
Besides the basic organization in nucleosome core particles (NCPs), eukaryotic chromatin is further packed through interactions with numerous protein complexes including transcription factors, chromatin remodeling and modifying enzymes. This nucleoprotein complex provides the template for many important biological processes, such as DNA replication, transcription, and DNA repair. Thus, to understand the molecular basis of these DNA transactions, it is critical to define individual changes of the chromatin structure at precise genomic regions where these machineries assemble and drive biological reactions. Single-molecule approaches provide the only possible solution to overcome the heterogenous nature of chromatin and monitor the behavior of individual chromatin transactions in real-time. In this review, we will give an overview of currently available single-molecule methods to obtain mechanistic insights into nucleosome positioning, histone modifications and DNA replication and transcription analysis-previously unattainable with population-based assays.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Review
Schlagwörter Dna Fiber Analysis ; Chromatin Accessibility ; Chromatin Replication/transcription ; Electron Microscopy ; Histone Modifications ; Magnetic/optical Tweezers ; Single-molecule Techniques ; Third-generation Sequencing; Rna-polymerase-ii; Lagging-strand Synthesis; Dna-replication; In-vivo; Transcription Initiation; Nucleosomal Barrier; Fork Reversal; Cross-linking; Human Genome; Cell-cycle
ISSN (print) / ISBN 2296-634X
e-ISSN 2296-634X
Quellenangaben Band: 9, Heft: , Seiten: , Artikelnummer: 699771 Supplement: ,
Verlag Frontiers
Verlagsort Lausanne
Begutachtungsstatus Peer reviewed
Förderungen European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program
Helmholtz Association