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Michalke, B. ; Berthele, A.* ; Venkataramani, V.*

Simultaneous quantification and speciation of trace metals in paired serum and CSF samples by size exclusion chromatography–inductively coupled plasma–dynamic reaction cell–mass spectrometry (SEC-DRC-ICP-MS).

Int. J. Mol. Sci. 22:8892 (2021)
Verlagsversion Forschungsdaten DOI
Open Access Gold
Creative Commons Lizenzvertrag
Background: Transition metals play a crucial role in brain metabolism: since they exist in different oxidation states they are involved in ROS generation, but they are also co-factors of enzymes in cellular energy metabolism or oxidative defense. Methods: Paired serum and cerebrospinal fluid (CSF) samples were analyzed for iron, zinc, copper and manganese as well as for speciation using SEC-ICP-DRC-MS. Brain extracts from Mn-exposed rats were additionally analyzed with SEC-ICP-DRC-MS. Results: The concentration patterns of transition metal size fractions were correlated between serum and CSF: Total element concentrations were significantly lower in CSF. Fe-ferritin was decreased in CSF whereas a LMW Fe fraction was relatively increased. The 400–600 kDa Zn fraction and the Cu-ceruloplasmin fraction were decreased in CSF, by contrast the 40–80 kDa fraction, containing Cu-and Zn-albumin, relatively increased. For manganese, the α-2-macroglobulin fraction showed significantly lower concentration in CSF, whereas the citrate Mn fraction was enriched. Results from the rat brain extracts supported the findings from human paired serum and CSF samples. Conclusions: Transition metals are strictly controlled at neural barriers (NB) of neurologic healthy patients. High molecular weight species are down-concentrated along NB, however, the Mn-citrate fraction seems to be less controlled, which may be problematic under environmental load.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Cerebrospinal Fluid ; Element Speciation ; Iron ; Manganese ; Paired Samples ; Sec-icp-drc-ms ; Serum; Anatomical Region Differences; Cerebrospinal-fluid; Zinc Homeostasis; Oxidative Stress; Manganese; Brain; Blood; Elements; Citrate; Beta
ISSN (print) / ISBN 1422-0067
e-ISSN 1661-6596
Quellenangaben Band: 22, Heft: 16, Seiten: , Artikelnummer: 8892 Supplement: ,
Verlag MDPI
Verlagsort Basel
Begutachtungsstatus Peer reviewed
Förderungen Deutsche Forschungsgemeinschaft