PuSH - Publication Server of Helmholtz Zentrum München

Madhavan, B.K.* ; Han, Z.* ; Sickmann, A.* ; Pepperkok, R.* ; Nawroth, P.P. ; Kumar, V.*

A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions.

STAR Protoc. 2:100700 (2021)
Publ. Version/Full Text DOI
Open Access Gold
Creative Commons Lizenzvertrag
With the advancement of laser-based microscopy tools, it is now possible to explore mechano-kinetic processes occurring inside the cell. Here, we describe the advanced protocol for studying the DNA repair kinetics in real time using the laser to induce the DNA damage. This protocol can be used for inducing, testing, and studying the repair mechanisms associated with DNA double-strand breaks, interstrand cross-link repair, and single-strand break repair. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2017, 2020).
Altmetric
Additional Metrics?
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Keywords Cancer ; Cell Biology ; Microscopy ; Molecular Biology
e-ISSN 2666-1667
Journal STAR Protocols
Quellenangaben Volume: 2, Issue: 3, Pages: , Article Number: 100700 Supplement: ,
Reviewing status Peer reviewed