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Crosbie, D.B.* ; Mahmoudi, M.* ; Radl, V. ; Brachmann, A.* ; Schloter, M. ; Kemen, E.* ; Marín, M.*

Microbiome profiling reveals that Pseudomonas antagonise parasitic nodule colonisation of cheater rhizobia in Lotus.

New Phytol. 234, 242-255 (2022)
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Open Access Gold (Paid Option)
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Nodule microbiota is dominated by symbiotic nitrogen-fixing rhizobia, however, other non-rhizobial bacteria also colonise this niche. Although many of these bacteria harbour plant-growth-promoting functions, it is not clear whether these less abundant nodule colonisers impact root-nodule symbiosis. We assessed the relationship between the nodule microbiome and nodulation as influenced by the soil microbiome, by using a metabarcoding approach to characterise the communities inside nodules of healthy and starved Lotus species. A machine learning algorithm and network analyses were used to identify nodule bacteria of interest, which were re-inoculated onto plants in controlled conditions to observe their potential functionality. The nodule microbiome of all tested species differed according to inoculum, but only that of Lotus burttii varied with plant health. Amplicon sequence variants representative of Pseudomonas species were the most indicative non-rhizobial signatures inside healthy L. burttii nodules and negatively correlated with Rhizobium sequences. A representative Pseudomonas isolate co-colonised nodules infected with a beneficial Mesorhizobium, but not with an ineffective Rhizobium isolate and another even reduced the number of ineffective nodules induced on Lotus japonicus. Our results show that nodule endophytes influence the overall outcome of the root-nodule symbiosis, albeit in a plant host-specific manner.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Lotus ; Mesorhizobium ; Pseudomonas ; Ineffective Nodules ; Root-nodule Symbiosis ; Microbiome; Plant-growth; Co-inoculation; Phaseolus-vulgaris; Nitrogen-fixation; Root; Nodulation; Legume; Strains; L.; Symbiosis
ISSN (print) / ISBN 0028-646X
e-ISSN 1469-8137
Journal New Phytologist
Quellenangaben Volume: 234, Issue: 1, Pages: 242-255 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place 111 River St, Hoboken 07030-5774, Nj Usa
Reviewing status Peer reviewed
Grants German Research Foundation (Deutsche Forschungsgemeinschaft (DFG)