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Brunet, T. ; Berutti, R. ; Dill, V.* ; Hecker, J.S.* ; Choukair, D.* ; Andres, S.* ; Deschauer, M.* ; Diehl-Schmid, J.* ; Krenn, M.* ; Eckstein, G. ; Graf, E.* ; Gasser, T.* ; Strom, T.M.* ; Hoefele, J.* ; Götze, K.S.* ; Meitinger, T.* ; Wagner, M.

Clonal hematopoiesis as a pitfall in germline variant interpretation in the context of Mendelian disorders.

Hum. Mol. Genet. 31, 2386-2395 (2022)
DOI
Free by publisher: Publ. Version/Full Text online available 06/2023
Clonal hematopoiesis due to somatic mutations in hematopoietic stem/progenitor cells is an age-related phenomenon and commonly observed when sequencing blood DNA in elderly individuals. Several genes that are implicated in clonal hematopoiesis are also associated with Mendelian disorders when mutated in the germline, potentially leading to variant misinterpretation. We performed a literature search to identify genes associated with age-related clonal hematopoiesis followed by an OMIM query to identify the subset of genes in which germline variants are associated with Mendelian disorders. We retrospectively screened for diagnostic cases in which the presence of age-related clonal hematopoiesis confounded exome sequencing data interpretation. We found 58 genes in which somatic mutations are implicated in clonal hematopoiesis while germline variants in the same genes are associated with Mendelian (mostly neurodevelopmental) disorders. Using five selected cases of individuals with suspected monogenic disorders, we illustrate how clonal hematopoiesis in either variant databases or exome sequencing datasets poses a pitfall, potentially leading to variant misclassification and erroneous conclusions regarding gene-disease associations.
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Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 0964-6906
e-ISSN 1460-2083
Quellenangaben Volume: 31, Issue: 14, Pages: 2386-2395 Article Number: , Supplement: ,
Publisher Oxford University Press
Reviewing status Peer reviewed