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Bandi, V.G.* ; Luciano, M.P.* ; Saccomano, M. ; Patel, N.L.* ; Bischof, T.S. ; Lingg, J.G.P. ; Tsrunchev,P. ; Nix, M.N.* ; Ruehle, B.* ; Sanders, C.* ; Riffle, L.* ; Robinson, C.M.* ; Difilippantonio, S.* ; Kalen, J.D.* ; Resch-Genger, U.* ; Ivanic, J.* ; Bruns, O.T. ; Schnermann, M.J.*

Targeted multicolor in vivo imaging over 1,000 nm enabled by nonamethine cyanines.

Nat. Methods 19, 353–358 (2022)
Open Access Green: Postprint online available 10/2022
Recent progress has shown that using wavelengths between 1,000 and 2,000 nm, referred to as the shortwave-infrared or near-infrared (NIR)-II range, can enable high-resolution in vivo imaging at depths not possible with conventional optical wavelengths. However, few bioconjugatable probes of the type that have proven invaluable for multiplexed imaging in the visible and NIR range are available for imaging these wavelengths. Using rational design, we have generated persulfonated indocyanine dyes with absorbance maxima at 872 and 1,072 nm through catechol-ring and aryl-ring fusion, respectively, onto the nonamethine scaffold. Multiplexed two-color and three-color in vivo imaging using monoclonal antibody and dextran conjugates in several tumor models illustrate the benefits of concurrent labeling of the tumor and healthy surrounding tissue and lymphatics. These efforts are enabled by complementary advances in a custom-built NIR/shortwave-infrared imaging setup and software package for multicolor real-time imaging.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Indocyanine Green; Fluorescence; Surgery; Labels; Dye
ISSN (print) / ISBN 1548-7091
e-ISSN 1548-7105
Journal Nature Methods
Quellenangaben Volume: 19, Issue: 3, Pages: 353–358 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place New York, NY
Reviewing status Peer reviewed
Institute(s) Helmholtz Pioneer Campus (HPC)
Grants Deutsche Forschungsgemeinschaft (German Research Foundation)
U.S. Department of Health & Human Services | National Institutes of Health (NIH)