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Visscher, M.* ; Pleitez, M.A. ; Van Gaalen, K.* ; Nieuwenhuizen-Bakker, I.M.* ; Ntziachristos, V. ; Van Soest, G.*

Label-free analytic histology of carotid atherosclerosis by mid-infrared optoacoustic microscopy.

Photoacoustics 26:100354 (2022)
Publ. Version/Full Text Research data DOI
Open Access Gold
Creative Commons Lizenzvertrag
Background and aims: Analysis of atherosclerotic plaque composition is a vital tool for unraveling the pathological metabolic processes that contribute to plaque growth. Methods: We visualize the constitution of human carotid plaques by mid-infrared optoacoustic microscopy (MiROM), a method for label-free analytic histology that requires minimal tissue preparation, rapidly yielding large field-of-view en-face images with a resolution of a few micrometers. We imaged endarterectomy specimens (n = 3, 12 sections total) at specific vibrational modes, targeting carbohydrates, lipids and proteins. Additionally, we recorded spectra at selected tissue locations. We identified correlations in the variability in this high-dimensional data set using non-negative matrix factorization (NMF). Results: We visualized high-risk plaque features with molecular assignment. Consistent NMF components relate to different dominant tissue constituents, dominated by lipids, proteins, and cholesterol and carbohydrates respectively. Conclusions: These results introduce MiROM as an innovative, stain-free, analytic histology technology for the biochemical characterization of complex human vascular pathology.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Atherosclerosis ; Cholesterol ; Mid-infrared Optoacoustic Microscopy ; Pathology ; Spectroscopy
ISSN (print) / ISBN 2213-5979
Journal Photoacoustics
Quellenangaben Volume: 26, Issue: , Pages: , Article Number: 100354 Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed
Grants Stichting Fonds Dr. Catharine van Tussenbroek and Nederlandse Organisatie voor Wetenschappelijk Onderzoek