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Untargeted and targeted circadian metabolomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and flow injection-electrospray ionization-tandem mass spectrometry (FIA-ESI-MS/MS).
In: Circadian Regulation. Berlin [u.a.]: Springer, 2022. 311-327 (Methods Mol. Biol. ; 2482)
A diverse array of 24-h oscillating hormones and metabolites direct and reflect circadian clock function. Circadian metabolomics uses advanced high-throughput analytical chemistry techniques to comprehensively profile these small molecules (<1.5 kDa) across 24 h in cells, media, body fluids, breath, tissues, and subcellular compartments. The goals of circadian metabolomics experiments are often multifaceted. These include identifying and tracking rhythmic metabolic inputs and outputs of central and peripheral circadian clocks, quantifying endogenous free-running period, monitoring relative phase alignment between clocks, and mapping pathophysiological consequences of clock disruption or misalignment. Depending on the particular experimental question, samples are collected under free-running or entrained conditions. Here we describe both untargeted and targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) and flow injection-electrospray ionization-tandem mass spectrometry (FIA-ESI-MS/MS) based assays we have used for circadian metabolomics studies. We discuss tissue homogenization, chemical derivatization, measurement, and tips for data processing, normalization, scaling, how to handle outliers, and imputation of missing values.
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Publication type Article: Edited volume or book chapter
Keywords Circadian ; Circadian Metabolomics ; Flow Injection-electrospray Ionization-tandem Mass Spectrometry (fia-esi-ms/ms) ; Liquid Chromatography-tandem Mass Spectrometry (lc-ms/ms) ; Metabolites ; Targeted Metabolomics ; Untargeted Metabolomics
ISSN (print) / ISBN 1064-3745
Book Volume Title Circadian Regulation
Journal Methods in Molecular Biology
Quellenangaben Volume: 2482, Pages: 311-327
Publishing Place Berlin [u.a.]
Reviewing status Peer reviewed