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Gabashvili, A.N.* ; Efremova, M.V. ; Vodopyanov, S.S.* ; Chmelyuk, N.S.* ; Oda, V.V.* ; Sarkisova, V.A.* ; Leonova, M.K.* ; Semkina, A.S.* ; Ivanova, A.V.* ; Abakumov, M.A.*

New approach to non-invasive tumor model monitoring via self-assemble iron containing protein nanocompartments.

Nanomaterials 12:1657 (2022)
Verlagsversion DOI
Open Access Gold
Creative Commons Lizenzvertrag
According to the World Health Organization, breast cancer is the most common oncological disease worldwide. There are multiple animal models for different types of breast carcinoma, allowing the research of tumor growth, metastasis, and angiogenesis. When studying these processes, it is crucial to visualize cancer cells for a prolonged time via a non-invasive method, for example, magnetic resonance imaging (MRI). In this study, we establish a new genetically encoded material based on Quasibacillus thermotolerans (Q.thermotolerans, Qt) encapsulin, stably expressed in mouse 4T1 breast carcinoma cells. The label consists of a protein shell containing an enzyme called ferroxidase. When adding Fe2+, a ferroxidase oxidizes Fe2+ to Fe3+, followed by iron oxide nanoparticles formation. Additionally, genes encoding mZip14 metal transporter, enhancing the iron transport, were inserted into the cells via lentiviral transduction. The expression of transgenic sequences does not affect cell viability, and the presence of magnetic nanoparticles formed inside encapsulins results in an increase in T2 relaxivity.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Encapsulins ; Magnetic Resonance Imaging ; Fluorescence ; Cell Tracking
ISSN (print) / ISBN 2079-4991
e-ISSN 2079-4991
Zeitschrift Nanomaterials
Quellenangaben Band: 12, Heft: 10, Seiten: , Artikelnummer: 1657 Supplement: ,
Verlag MDPI
Verlagsort Basel
Begutachtungsstatus Peer reviewed
Institut(e) Insitute of Synthetic Biomedicine (ISBM)