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Volumetric real-time multispectral optoacoustic tomography of biomarkers.
Nat. Protoc. 6, 1121-1129 (2011)
Multispectral optoacoustic tomography (MSOT) has recently been developed to enable visualization of optical contrast and tissue biomarkers, with resolution and speed representative of ultrasound. In the implementation described here, MSOT enables operation in real-time mode by capturing single cross-sectional images in <1 ms from living small animals (e.g., mice) and other tissues of similar dimensions. At the core of the method is illumination of the object using multiple wavelengths in order to resolve spectrally distinct biomarkers over background tissue chromophores. The system allows horizontal placement of a mouse in the imaging chamber and three-dimensional scanning of the entire body without the need to immerse the mouse in water. Here we provide a detailed description of the MSOT scanner components, system calibration, selection of image reconstruction algorithms and animal handling. Overall, the entire protocol can be completed within 15-30 min for acquisition of a whole-body multispectral data set from a living mouse.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Functional photoacoustic microscopy; In-vivo; Small animals; Resolution; Algorithm; Plaques; System; Brain; Array: ; MSOT
ISSN (print) / ISBN 1754-2189
Journal Nature Protocols
Quellenangaben Volume: 6, Issue: 8, Pages: 1121-1129
Publisher Nature Publishing Group
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)