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Randriamanantsoa, S.* ; Papargyriou, A. ; Maurer, H.C.* ; Peschke, K.* ; Schuster, M.* ; Zecchin, G.* ; Steiger, K.* ; Öllinger, R.* ; Saur, D.* ; Scheel, C. ; Rad, R.* ; Hannezo, E.* ; Bausch, A.R.*

Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids.

Nat. Commun. 13:5219 (2022)
Verlagsversion Forschungsdaten Forschungsdaten Forschungsdaten DOI
Open Access Gold
Creative Commons Lizenzvertrag
The development dynamics and self-organization of glandular branched epithelia is of utmost importance for our understanding of diverse processes ranging from normal tissue growth to the growth of cancerous tissues. Using single primary murine pancreatic ductal adenocarcinoma (PDAC) cells embedded in a collagen matrix and adapted media supplementation, we generate organoids that self-organize into highly branched structures displaying a seamless lumen connecting terminal end buds, replicating in vivo PDAC architecture. We identify distinct morphogenesis phases, each characterized by a unique pattern of cell invasion, matrix deformation, protein expression, and respective molecular dependencies. We propose a minimal theoretical model of a branching and proliferating tissue, capturing the dynamics of the first phases. Observing the interaction of morphogenesis, mechanical environment and gene expression in vitro sets a benchmark for the understanding of self-organization processes governing complex organoid structure formation processes and branching morphogenesis.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Zeitschrift Nature Communications
Quellenangaben Band: 13, Heft: 1, Seiten: , Artikelnummer: 5219 Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Begutachtungsstatus Peer reviewed
Förderungen Deutsche Forschungsgemeinschaft (German Research Foundation)