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Transplantation of CD6-depleted peripheral blood stem cells after DLA-haploidentical bone marrow transplantation contributes to engraftment and tolerance in a preclinical model of stem cell transplantation.

Vet. Immunol. Immunopathol. 144, 27-35 (2011)
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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Human leukocyte antigen (HLA)-haploidentical stem cell transplantation is an opportunity for nearly all patients lacking an HLA matched stem cell donor. However, graft rejection and graft-versus-host disease (GvHD) as well as infectious complications still result in high treatment-related mortality. Here, we used the dog as a preclinical model for the study of tolerance induction with the aim to optimize and to improve a clinical protocol of haploidentical stem cell transplantation. For this purpose CD6-depleted peripheral blood stem cells (PBSCs) were transfused 6d after transplantation of unmodified bone marrow from dog leukocyte antigen (DLA)-haploidentical littermate donors in order to induce immune tolerance. Besides hematopoietic stem cells CD6-depleted PBSC contain, NK cells and a minority of suppressive CD8-positive cells that may suppress activated T lymphocytes. Recipients were conditioned with, cyclophosphamide and antithymocyte globulin (ATG) preceded by a transfusion of donor buffy coat and either 1, 2 or 3×3.3Gy total body irradiation (TBI). Postgrafting immunosuppression was limited to 30d of cyclosporine and methotrexate. The additional administration of CD6-depleted PBSCs after unmodified marrow could not prevent GvHD, but it may improve engraftment and chimerism after conditioning with 2×3.3Gy TBI. Reasons for incomplete suppression and possible improvements for clinical applications are discussed.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Haploidentical stem cell transplantation; CD6-depletion; Dog; Animal model; GvHD; Tolerance
ISSN (print) / ISBN 0165-2427
e-ISSN 1873-2534
Quellenangaben Band: 144, Heft: 1-2, Seiten: 27-35 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Amsterdam, Netherlands
Begutachtungsstatus Peer reviewed