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Next generation sequencing and syntenic integration of flow-sorted arms of wheat chromosome 4A exposes the chromosome structure and gene content.
Plant J. 69, 377-386 (2012)
Wheat is the third most important crop for human nutrition in the world. The availability of high resolution genetic and physical maps and ultimately a complete genome sequence holds great promises for breeding improved varieties to cope with increasing food demand under the conditions of changing global climate. However, the large size of the bread wheat genome (∼17 Gb/1C) and the triplication of genic sequence resulting from its hexaploid status together impede the genome sequencing of this important crop species. Here we describe the application of mitotic chromosome flow sorting to separately purify and then shotgun sequence a pair of telocentric chromosomes which together form chromosome 4A (856 Mb/1C) of wheat. The isolation of this much reduced template and the consequent avoidance of the problem of sequence duplication, in conjunction with synteny-based comparisons with other grass genomes, have facilitated the construction of an ordered gene map of chromosome 4A, embracing ≥85% of its total gene content. It has also allowed for the precise localization of the various translocation and inversion breakpoints on chromosome 4A which differentiate it from its progenitor chromosome in the A genome diploid donor. The gene map of 4A along with the emerging sequences of homoeologous wheat chromosome groups 4, 5, and 7 represent unique resources that will, among other, allow to generate new insights into evolutionary dynamics between the homoeologous chromosomes and syntenic chromosomal regions.
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Publication type Article: Journal article
Document type Scientific Article
Keywords wheat genome; chromosome sorting; genome zipper; grass comparative genomics; wheat shotgun chromosome; Triticeae genome; TRITICUM-AESTIVUM L.; QUANTITATIVE TRAIT LOCI; BREAD WHEAT; AGRONOMIC TRAITS; GENOME EVOLUTION; B-GENOME; RESISTANCE; BARLEY; MAP; IDENTIFICATION