PuSH - Publication Server of Helmholtz Zentrum München

Advances in tracking hematopoiesis at the single-cell level.

Curr. Opin. Hematol. 19, 243-249 (2012)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
PURPOSE OF REVIEW: Studying heterogeneous populations, such as hematopoietic stem cells (HSCs), requires continuous long-term observation of living cells at the single-cell level. The purpose of this review is to discuss recent advances in technologies required for continuous single-cell analysis and the contribution of this approach to find answers in hematopoiesis research. RECENT FINDINGS: Continuous long-term imaging at the single-cell level still requires custom-made hardware, software and manual in-depth analysis of large amounts of data. Despite these technical difficulties, continuous time-lapse imaging and single-cell tracking are increasingly used in hematopoiesis research. It has already contributed to answering decades-old questions. SUMMARY: Continuous long-term single-cell analysis is indispensable for a comprehensive analysis of dynamic processes in heterogeneous cell populations. Despite many remaining technological hurdles, this approach is increasingly used in hematopoiesis research.
Altmetric
Additional Metrics?
Edit extra informations Login
Publication type Article: Journal article
Document type Review
Keywords Cell Tracking; Continuous Single-cell Imaging; Hematopoiesis; Stem Cells; STEM-CELLS; LONG-TERM; BONE-MARROW; AORTIC ENDOTHELIUM; SELF-RENEWAL; IN-VIVO; NICHE; DIFFERENTIATION; IDENTIFICATION; BEHAVIOR
Reviewing status