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Advances in tracking hematopoiesis at the single-cell level.
Curr. Opin. Hematol. 19, 243-249 (2012)
PURPOSE OF REVIEW: Studying heterogeneous populations, such as hematopoietic stem cells (HSCs), requires continuous long-term observation of living cells at the single-cell level. The purpose of this review is to discuss recent advances in technologies required for continuous single-cell analysis and the contribution of this approach to find answers in hematopoiesis research. RECENT FINDINGS: Continuous long-term imaging at the single-cell level still requires custom-made hardware, software and manual in-depth analysis of large amounts of data. Despite these technical difficulties, continuous time-lapse imaging and single-cell tracking are increasingly used in hematopoiesis research. It has already contributed to answering decades-old questions. SUMMARY: Continuous long-term single-cell analysis is indispensable for a comprehensive analysis of dynamic processes in heterogeneous cell populations. Despite many remaining technological hurdles, this approach is increasingly used in hematopoiesis research.
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Publication type Article: Journal article
Document type Review
Keywords Cell Tracking; Continuous Single-cell Imaging; Hematopoiesis; Stem Cells; STEM-CELLS; LONG-TERM; BONE-MARROW; AORTIC ENDOTHELIUM; SELF-RENEWAL; IN-VIVO; NICHE; DIFFERENTIATION; IDENTIFICATION; BEHAVIOR
Institute(s) Research Unit Stem Cell Dynamics (SCD)