PuSH - Publikationsserver des Helmholtz Zentrums München

Drukker, M. ; Tang, C.* ; Ardehali, R.* ; Rinkevich, Y.* ; Seita, J.* ; Lee, A.S.* ; Mosley, A.R.* ; Weissman, I.L.* ; Soen, Y.*

Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.

Nat. Biotechnol. 30, 531-542 (2012)
Verlagsversion Volltext DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
To identify early populations of committed progenitors derived from human embryonic stem cells (hESCs), we screened self-renewing, BMP4-treated and retinoic acid-treated cultures with >400 antibodies recognizing cell-surface antigens. Sorting of >30 subpopulations followed by transcriptional analysis of developmental genes identified four distinct candidate progenitor groups. Subsets detected in self-renewing cultures, including CXCR4(+) cells, expressed primitive endoderm genes. Expression of Cxcr4 in primitive endoderm was confirmed in visceral endoderm of mouse embryos. BMP4-induced progenitors exhibited gene signatures of mesoderm, trophoblast and vascular endothelium, suggesting correspondence to gastrulation-stage primitive streak, chorion and allantois precursors, respectively. Functional studies in vitro and in vivo confirmed that ROR2(+) cells produce mesoderm progeny, APA(+) cells generate syncytiotrophoblasts and CD87(+) cells give rise to vasculature. The same progenitor classes emerged during the differentiation of human induced pluripotent stem cells (hiPSCs). These markers and progenitors provide tools for purifying human tissue-regenerating progenitors and for studying the commitment of pluripotent stem cells to lineage progenitors.
Altmetric
Weitere Metriken?
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter BONE MORPHOGENETIC PROTEIN-4; RECESSIVE ROBINOW-SYNDROME; HUMAN PLACENTA; MOUSE EMBRYO; TRANSCRIPTION FACTOR; DEFINITIVE ENDODERM; VISCERAL ENDODERM; IN-VIVO; EXPRESSION; DIFFERENTIATION
ISSN (print) / ISBN 0733-222X
e-ISSN 1546-1696
Zeitschrift Nature Biotechnology
Quellenangaben Band: 30, Heft: 6, Seiten: 531-542 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort New York, NY
Begutachtungsstatus Peer reviewed