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Saraste, A.* ; Laitinen, I.* ; Weidl, E.* ; Wildgruber, M.* ; Weber, A.W.* ; Nekolla, S.G.* ; Hölzlwimmer, G. ; Esposito, I. ; Walch, A.K. ; Leppänen, P.* ; Lisinen, I.* ; Luppa, P.B.* ; Yla-Herttuala, S.* ; Wester, H.J.* ; Knuuti, J.* ; Schwaiger, M.*

Diet intervention reduces uptake of αvβ3 integrin-targeted PET tracer 18F-galacto-RGD in mouse atherosclerotic plaques.

J. Nucl. Cardiol. 19, 775-784 (2012)
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Expression of alpha(v)beta(3) integrin has been proposed as a marker for atherosclerotic lesion inflammation. We studied whether diet intervention reduces uptake of alpha(v)beta(3) integrin-targeted positron emission tomography tracer F-18-galacto-RGD in mouse atherosclerotic plaques. Hypercholesterolemic LDLR-/- ApoB(100/100) mice on high-fat diet for 4 months were randomized to further 3 months on high-fat diet (high-fat group, n = 8) or regular mouse chow (intervention group, n = 7). Intima-media ratio describing plaque burden was comparable between intervention and high-fat groups (2.0 +/- A 0.5 vs 2.3 +/- A 0.8, P = .5). Uptake of F-18-galacto-RGD in the aorta was lower in the intervention than high-fat group (%ID/g 0.16 vs 0.23, P < .01). Autoradiography showed 35% lower uptake of F-18-galacto-RGD in the atherosclerotic plaques in the intervention than high-fat group (P = .007). Uptake of F-18-galacto-RGD in plaques correlated with uptake of H-3-deoxyglucose and nuclear density, which was lower in the intervention than high-fat group (P = .01). Flow cytometry demonstrated macrophages expressing alpha(v) and beta(3) integrins in the aorta. Uptake of F-18-galacto-RGD in mouse atherosclerotic lesions was reduced by lipid-lowering diet intervention. Expression of alpha(v)beta(3) integrin is a potential target for evaluation of therapy response in atherosclerosis.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Atherosclerosis ; Pet ; Inflammation ; Molecular Imaging ; Vulnerable Plaque; POSITRON-EMISSION-TOMOGRAPHY; ALPHA-V-BETA-3 INTEGRIN; INFLAMMATION; MICE; HYPERCHOLESTEROLEMIA; NANOPARTICLES; ANGIOGENESIS; EXPRESSION; MODEL
ISSN (print) / ISBN 1071-3581
e-ISSN 1532-6551
Zeitschrift Journal of Nuclear Cardiology
Quellenangaben Band: 19, Heft: 4, Seiten: 775-784 Artikelnummer: , Supplement: ,
Verlag Springer
Begutachtungsstatus