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Graf, C.* ; Kuehne, C.* ; Panhuysen, M.* ; Puetz, B.* ; Weber, P.* ; Holsboer, F.* ; Wurst, W. ; Deussing, J.M.*

Corticotropin-releasing hormone regulates common target genes with divergent functions in corticotrope and neuronal cells.

Mol. Cell. Endocrinol. 362, 29-38 (2012)
Verlagsversion Volltext DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
As a key regulator of the neuroendocrine stress axis and as a neuromodulator in the brain, the neuropeptide corticotropin-releasing hormone (CRH) plays an important role in various diseases of the central nervous system. Its cognate receptor CRH receptor type 1 (CRHR1) is a potential novel target for the therapeutic intervention in major depressive disorder. Therefore, a more precise understanding of involved intracellular signaling mechanisms is essential. The objective of this project was to identify specific target genes of CRHR1-mediated signaling pathways in the corticotrope cell line AtT-20 and in the neuronal cell line HN9 using microarray technology and qRT-PCR, respectively. In addition, we assessed the capacity of validated target genes to directly impact on CRHR1-dependent signaling using reporter assays. Thereby, we identified a set of CRHR1 downstream targets with diverging and cell type-specific roles which strengthen the role of CRH and CRHR1 as dynamic modulators of a variety of signal transduction mechanisms and cellular processes.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Corticotropin-releasing hormone; HPA axis; Expression profiling; AtT-20; CRHR1
ISSN (print) / ISBN 0303-7207
e-ISSN 1872-8057
Quellenangaben Band: 362, Heft: 1-2, Seiten: 29-38 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Shannon
Begutachtungsstatus Peer reviewed