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Improved protocol for the extraction of bacterial mRNA from soils.
J. Microbiol. Methods 91, 62-64 (2012)
An improved protocol for extraction of prokaryotic mRNA from soil samples was developed by modifying the extraction procedure to obtain higher yields of mRNA and to reduce co-extraction of humic acids. The modified protocol was found to be more robust and efficient compared to the original protocol by Griffiths et al. (2000) without compromising with the quality and quantity of RNA.
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Publication type Article: Journal article
Document type Scientific Article
Keywords mRNA; Soil; glnA
Institute(s) Research Unit Comparative Microbiome Analysis (COMI)