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Construction and isolation of recombinant MVA.

Methods Mol. Biol. 269, 77-99 (2004)
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Modified vaccinia virus Ankara (MVA) is a valuable tool for the expression of recombinant genes used for such purposes as the study of protein functions or characterization of cellular and humoral immune responses. A major advantage of MVA is its clear safety record, and it can be handled under biosafety level 1 conditions. Despite its replication deficiency in human and most mammalian cells, MVA provides high-level gene expression and has proven to be immunogenic when delivering heterologous antigens in animals and humans. This chapter provides state-of-the-art protocols for generation, plaque isolation, molecular characterization, as well as amplification and purification of MVA vector viruses to obtain recombinant viruses for further evaluation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Herausgeber Isaacs, S.N.*
Schlagwörter poxvirus; modified vaccinia virus Ankara (MVA); recombinant vaccine
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Konferenztitel Vaccinia Virus and Poxvirology: Methods and Protocols
Quellenangaben Band: 269, Heft: , Seiten: 77-99 Artikelnummer: , Supplement: ,
Reihe Methods in Molecular Biology
Verlag Springer
Verlagsort Berlin [u.a.]
Begutachtungsstatus Peer reviewed